EBSeq: an empirical Bayes hierarchical model for inference in RNA-seq experiments

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EBSeq: an empirical Bayes hierarchical model for inference in RNA-seq experiments

MOTIVATION Messenger RNA expression is important in normal development and differentiation, as well as in manifestation of disease. RNA-seq experiments allow for the identification of differentially expressed (DE) genes and their corresponding isoforms on a genome-wide scale. However, statistical methods are required to ensure that accurate identifications are made. A number of methods exist fo...

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A two-factor experiment with interaction between factors wherein observations follow an Inverse Gaussian model is considered. Analysis of the experiment is approached via an empirical Bayes procedure. The conjugate family of prior distributions is considered. Bayes and empirical Bayes estimators are derived. Application of the procedure is illustrated on a data set, which has previously been an...

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ژورنال

عنوان ژورنال: Bioinformatics

سال: 2013

ISSN: 1460-2059,1367-4803

DOI: 10.1093/bioinformatics/btt087